BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1847

Peptides:

Sequences
CTGSTQHQC CHSALTKHC CSTHFIDTC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21903933

Target Name:

Porcine skin

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Screening studies were performed on porcine skin in Franz diffusion cells (FDCs, PermeGear). The phage display library was placed in the donor compartment of the FDC. After 24 h, phage which had penetrated through the skin were collected from the receiver compartment, amplified, and again placed in the donor compartment for an additional round of screening to further narrow down the library.

BiopanningDataSet Comments:

Peptides that penetrate the SC were identified using in vitro phage display, which were labeled a skin permeating and cell entering (SPACE) peptide. In vitro studies indicated that the SPACE peptide, when conjugated to cargoes such as small molecules and proteins, was able to facilitate their penetration across the SC into epidermis and dermis.

Curator:

Curation Date:

0000-00-00

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