BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1844

Peptides:

Sequences
HWGNHSKSHPQR(3) HTLHRQVPKHWL(2) HYQHNTHHPSRW(2) HSSSASDRSRPL(1) STHHRHYHDTLA(1) GHIHSMRHHRPT(2) KHMHWHPPALNT(28)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21856287

Target Name:

PreS1 protein

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Alignment of the positive phage clones revealed a putative consensus PreS1 binding motif of H-(X)n-H-(X)m-H-P/R. Moreover, a peptide named P7 (KHMHWHPPALNT) was highly enriched and occurred with a surprisingly high frequency of 72%. A thermodynamic study revealed that P7 has a higher binding affinity to PreS1 than the other peptides. Furthermore, P7 was able to abrogate the binding of HBV virions to the PreS1 antibody, suggesting that P7 covers key functional sites on the native PreS1 protein. This newly isolated peptide may, therefore, be a new therapeutic candidate for the treatment of HBV.

Curator:

Curation Date:

0000-00-00

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