BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1842

Peptides:

Sequences
CLRSHPLGC CTFASVMTC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21829538

Target Name:

Soluble ectodomain of APP isoform sAPPα695

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Five out of 28 individually picked sAPPa-binding clones contained the peptide sequence LRSHPLG. An additional sAPPa-binding clone comprising amino acid sequence TFASVMT was isolated. The sequences of both peptides were then compared to human protein sequences in the NCBI databank using BLAST. This analysis revealed that both peptides presented significant sequence homologies to several members of the human semaphorin protein family. Interestingly, the TFASVMT peptide is homologous to a region of semaphorin 3A that has been shown to be important for receptor recognition, while the region that is homologous to the LRSHPLG peptide lies very close to that same important site. These initial results suggested that sAPPa binds to semaphorins at a domain that is important for receptor recognition and biological activity.

Curator:

Curation Date:

0000-00-00

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