BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1824

Peptides:

Sequences
ATWAVL(1) AVMTSS(1) SWFLQW(1) REWISH(1) YTALLI(1) CYLCSV(1) LFSSGK(1) VWHLLH(1) VPWWVP(1) LSRILF(1) PGHSPW(1) WNLRSS(1) IALMDY(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 7682645

Target Name:

Insulin

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f3-6mer phage display library (X6)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The difference between A405 and A495 is taken as the signal. The strongest signals amount to ~0.6, while background is ~0.02. Only relatively strong ligands give signals in this assay.

Brief Description:

Phages displaying a ligand for the biotinylated insulin are captured on the dish by binding of the biotin moiety to immobilized streptavidin.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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