BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1803

Peptides:

Sequences	Affinity Values
WHWRLPS(10) HKPLQIW(5) QAHTVGK(4) YVHRLPP(2) HYTRHSA(1)	[2.290 ± 0.094] [0.363 ± 0.088] [0.834 ± 0.451] [0.555 ± 0.078] [0.373 ± 0.114]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16413648

Target Name:

Microcystin-LR, MC-LR

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Affinities for MC-LR of the five different selected clones were measured by ELISA. Absorbances at 410 nm were reproduced from the bar graph and expressed as the means of three determinations ± sample standard deviations. L7 represented original linear 7 peptides library and its absorbance was 0.256 ± 0.266. Clone L7-3 (displaying WHWRLPS) expressed the highest affinity for MC-LR as compared to the original library and their negative controls.

Brief Description:

BiopanningDataSet Comments:

The affinity of selected phage to MC-LR were tested through an immunoprecipitation assay. The results showed that all selected phage combined with MC-LR except the YVHRLPP-bearing phage.

Curator:

Curation Date:

0000-00-00

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