BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1787

Peptides:

Sequences	Affinity Values
VAWCTIFLCLDV ADFCEGKDMIDWVYCRLY FWFCDRIAWYPQHLCEFL FRNCEPWMLRFGCNPR	[0.22] [2.5] [1.9] [4.8]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11934284

Target Name:

Human serum albumin

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

TN phage display library pool

Affinity Measurement Method:

Fluorescence anisotropy

Affinity Measurement Description:

Fluorescence anisotropy measurements were performed in various conditions. It was discovered that many of the peptides bound better at a lower pH (3 mM phosphate, pH 6.2) and in the absence of added salt. The dissociation constants (KD, μM) of this condition were shown.

Brief Description:

BiopanningDataSet Comments:

The pooled phage libraries of TN6-6, TN10-9, and TN12-1 were used in selections against human serum albumin (HSA) immobilized directly on polystyrene microtiter plates. After two rounds of selection, phage peptide selection on directly immobilized HSA yielded several phage isolates (232, 234, 236, and 238) that showed positive HSA binding by ELISA. Phage isolate 232 (VAWCTIFLCLDV) showed the highest ELISA signal. Although in solution DX-232 bound HSA with the highest affinity, the DX-232-Sepharose column performance was bad, binding no detectable HSA. In contrast, the DX-236(FWFCDRIAWYPQHLCEFL)-Sepharose column was the best performer and quantitatively bound the entire 1 mg injection (0.15 μmol). At higher HSA loads in the no salt, pH 6.2 buffer, the DX-236-Sepharose column (0.35 mL) bound upward of 4 mg of HSA, which corresponds to a dynamic capacity of greater than 11 mg/mL. The DX-236 affinity column bound HSA from human serum with a greater specificity than does Cibacron Blue agarose beads. DX-236 also bound well to several mammalian serum albumins.

Curator:

Curation Date:

0000-00-00

First |

Prev |

1787

| Next

| Last