BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1784

Peptides:

Sequences	Affinity Values
NACYVDLFLGASVCP(0.46) SSAKSHCYAFCSGLP(0.31) HCRKVTGSDYLLCGL(0.05)	[0.07] [0.25] [0.05]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10600340

Target Name:

IgG from the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f88-15mer phage display library (X15)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

IgG immunoreactivity of MS sera against the selected phage-displayed peptides was detected by ELISA on immobilized phages with a pool of five MS sera diluted to 1:100. The results are expressed as the difference between the OD obtained at 492 nm with MS sera and that with non-MS sera.

Brief Description:

The serum (20 ml, about 200 mg of IgG) from multiple sclerosis patients was used as the target, which was imobilized on to the streptavidin-coated polystyrene petri dishes through biotinylated mouse anti-human IgG monoclonal antibody P5F2F7. For each round of panning, serum from a different multiple sclerosis patient was used. In the fourth round, the amplified eluate was incubated with 100 ml of a pool of sera from five non-multiple sclerosis patients before reacting with dish-bound serum IgG from multiple sclerosis patient.

BiopanningDataSet Comments:

Thirty-six clones were randomly picked and sequenced. Compared to control peptide, NACYVDLFLGASVCP and HCRKVTGSDYLLCGL were not specifically recognized by individual multiple sclerosis sera whereas SSAKSHCYAFCSGLP was significantly recognized by only 3 of 37 sera.

Curator:

Curation Date:

0000-00-00

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