BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1763

Peptides:

Sequences	Affinity Values
DFRRLPGAFWQLRQP(13) GWWYKGRARPVSAVA(4) VWRLLAPPFSNRLLP(1)	[0.429, 24][0.2] [0.398, 13][0.5] [0.488, 1][0.2]

Sequences Features:

Motif:

R-x-L-P-x(2)-F-x(3)-R-x-P

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10456319

Target Name:

Ganglioside GM1

Template Name:

Cholera toxin B protein, CTB

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 fUSE5 phage display library

Affinity Measurement Method:

ELISA,Quartz crystal microbalance (QCM)

Affinity Measurement Description:

In phage ELISA, the absorbance at 492 nm was determined. Absorbances were reproduced from the graph when the concentration of phage was 5 nM. And the value of the control phage (displaying LGRAGQSYPSFARGL) was 0.221. Besides, in binding inhibition assay, horseradish peroxidase-conjugated CTB and synthetic peptides were incubated with the GM1 plates in a 24 well plate. The IC50 values (μM) of synthetic peptides were determined and shown. Furthermore the dissociation constants (Kd values, nM) were determined by QCM and shown.

Brief Description:

BiopanningDataSet Comments:

Three synthetic pentadecapeptides inhibited the binding of cholera toxin B subunit to the GM1 monolayer with an IC50 of 24, 13 and 1.0 μM, respectively. These peptides will be useful for searching functional roles of ganglioside GM1.

Curator:

Curation Date:

0000-00-00

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