BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 168

Peptides:

Sequences

Affinity Values

RDFYYN(5)
HDFFFN(2)
WDLWVN(1)
PGRYIN(1)
NNYFFV(1)
GSGSIN(1)
MPSYIN(1)
PPAYFN(1)
PDLYIN(1)
MPQYVN(1)
RGFYYN(1)
NNYFFN(1)
ARMYIN(1)
DDTYNS(1)
RGLYYN(1)

[+][NT]
[+][5.1 ± 0.3]
[+][3.4 ± 0.2]
[+][5.7 ± 1.1]
[+][0.006]
[+][0.9 ± 0.1]
[+][3.5 ± 0.2]
[+][1.8 ± 0.5]
[+][4.5 ± 0.1]
[+][2.0 ± 0.6]
[+][6.6 ± 1.6]
[+][6.6 ± 1.0]
[+][4.0 ± 0.2]
[+][1.8 ± 0.2]
[+][5.6 ± 0.1]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 7499368

Target Name:

Cardiac glycoside digoxin

Template Name:

Anti-digoxin antibody 26-10 Fab

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X6 phage display library

Affinity Measurement Method:

ELISA,Saturation equilibrium assay

Affinity Measurement Description:

Secreted Fab was tested using an ELISA with goat anti-mouse Fab, digoxin-BSA, and the congener-BSA used for selection as coating antigens. All clones were positive for Fab. Besides the designation + or - refers to whether the clone binds to the congener used in selection. Affinities (Ka,e-9 M-1) for digoxin of Fab 26 -10 and mutants (randomly mutated at positions H30 -35 inclusive) selected from the bacteriophage library. Affinities were measured using an equilibrium saturation method with filtration through glass fiber filters for separation of bound and free ligand, except that Fab was immobilized on the filter using goat anti-mouse Fab.

Brief Description:

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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