BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1674

Peptides:

Sequences
MDAQTRRRERRAMERAQTSSSL(1) MDAQTRRRERRAMERATLPQVL(11) MDAQTRRRERRAALRNEKFWVV(1) MDAQTRRRERRALMRRNARIAV(1) MDAQTRRRERRARDRFRLLRTL(1) MNAQTRRRERRALAFKARYMAL(1) MDAQTRRRERRAWERRTQSWMV(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

mRNA display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11675487

Target Name:

Three RNA targets (λ-boxBR, GNRA tetraloop, and P22 boxBL)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X10 mRNA display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Each peptide tested could bind at least one of the hairpins in the mixture. The specificity of seven individual clones, the round 11 and round 12 pools were then tested by using each individual hairpin. All showed a marked preference for the boxBR RNA as compared with the GNRA or P22 targets.

Curator:

Curation Date:

0000-00-00

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