BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1668

Peptides:

Sequences
MVMLACRATKML(3) MWMTSCRATKML(1) MAFFGCRATKML(1) MPGARCRATKML(1) MLGQHCRATKML(1) MLRGFCRATKML(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

mRNA display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16122707

Target Name:

Botulinum neurotoxin A light chain

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

M-X(4)-CRATKML mRNA display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Purified biotin-ubiquitin BoNT/A LC immobilized on Softlink monomeric avidin (Promega) was first incubated with the RNA-peptides for specified time. RNA-peptide fusions bound to the immobilized BoNT/A LC were eluted off the column with 5 mM biotin in the same buffer.

BiopanningDataSet Comments:

A protease assay was developed using immobilized intact SNAP-25 as the substrate. The new peptide inhibitors showed a 10-fold increase in affinity to BoNT/A light chain than the parent peptide. Interestingly, the sequences of the new peptide inhibitors showed abundant hydrophobic residues but few hydrophilic residues.

Curator:

Curation Date:

0000-00-00

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