BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1635

Peptides:

Sequences

Affinity Values

SHRLHAT*ILMP(1)
SHRILSSALV*V(1)
SHRLHNTMPSES(1)
THRLYLASTLPG(1)
SHRLPHPNLIRL(1)
QHRIHNPAPVSL(1)
HHRILIEPPSMH(1)
SHRLPPVSNPSL(1)
HHRLNNQDDLLK(1)
SHKLFYQMGTQL(1)
QHRMTWMQGLSS(1)
QHKLYPVIPLPS(1)

[1.680]
[1.530]
[2.006]
[1.685]
[1.830]
[2.083]
[1.685]
[1.607]
[1.860]
[1.573]
[1.685]
[2.100]

Sequences Features:

Motif:

H-[RK]-[LI]

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 13679612

Target Name:

Anti-DENV2 monoclonal antibody Ab4

Template Name:

Dengue virus 2, DENV2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X12 phage display library

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

In phage ELISA, the absorbance at 490 nm was measured. Data shown were reproduced from the graph.

Brief Description:

BiopanningDataSet Comments:

Selected phage clones reacted specifically with Ab4 and did not react with other mAbs. Immunopositive phage clones displayed a consensus motif, His-Arg/Lys-Leu/Ile, and a synthetic peptide corresponding to the phage-displayed peptide bound specifically to Ab4. The His and Arg residues in this epitope were found to be crucial for peptide binding to Ab4 and binding activity decreased dramatically when these residues were changed to Leu. The epitope-based synthetic peptide not only identified serum samples from DEN-2-immunized mice and rabbits by ELISA but also differentiated clearly between serum samples from DEN-2- and Japanese encephalitis virus-immunized mice.

Curator:

Curation Date:

0000-00-00

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