BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1624

Peptides:

Sequences
TYNHDYKTGMVIYSPFMTYP

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21697352

Target Name:

Anti-NSP2 monoclonal antibody

Template Name:

Non-structural protein 2, NSP2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X20 fUSE5 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

BLAST and ClustalW2 analysis of the phage clone TYNHDYKTGMVIYSPFMTYP peptide and NSP2 protein sequences located a motif (244)T-(Y/F)-Φ-Φ-Φ-X-K-Φ-G(252), in the C-terminal domain of the protein, where Φ is a hydrophilic residue and amino acids at position X249 did not display conserved/similar properties. This motif was found to be conserved from analyzing an alignment of Australian and GenBank human NSP2 sequences, which also suggests that antibodies against NSP2 may provide heterotypic protection.

Curator:

Curation Date:

0000-00-00

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