BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1621

Peptides:

Sequences

Affinity Values

RIGRPLR(1)
GGKRPAR(1)
FNRKERR(1)
GKGRAQR(1)
SRAKKPR(1)
NGARKPR(1)
LRKRAAR(1)
KKVRVVR(1)
GKVGSRR(1)
VYKARTR(1)
LSSAQDR(1)
VGSGKRS(1)
SMTARSV(1)
GGRGARA(1)
RVRAGHH(1)

[5567, 0.78]
[4645, 1.51]
[2650, NT]
[1045, NT]
[912, NT]
[817, 3.29]
[581, NT]
[474, NT]
[377, NT]
[293, 10.9]
[14, NT]
[1.4, NT]
[0.8, NT]
[0.6, NT]
[0.8, NT]

Sequences Features:

Motif:

[RK]-x(2)-R

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21486998

Target Name:

Neuropilin-1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X7 T7 phage display library

Affinity Measurement Method:

Competition experiment

Affinity Measurement Description:

Fifteen phage clones were randomly picked from the R3 pool and their binding to PPC-1 relative to M21 melanoma cells was measured. Of note, in contrast to PPC-1 cells, M21 cells express only minimal amounts of NRP-1 protein. The binding fold was shown. Besides, the equilibrium dissociation constants (Kd, μM) of the peptide sequences for binding to the NRP-1 protein were measured and shown. Three rounds of selection in MiPS device revealed peptides P7 (RIGRPLR) and P4 (GGKRPAR) exhibiting higher affinity and specificity in binding to cells expressing NRP-1 compared with the well known CR (RPARPAR) with a Kd of 2.79 μM. NT denotes not tested.

Brief Description:

PPC-1 cells were selected as a model because they express high levels of NRP-1. Selection was performed against PPC-1 cells by the microfluidic phage selection (MiPS) platform. The ability of this system to directly target membrane-bound proteins on cell surfaces is significant because most of these proteins are notoriously difficult to produce using recombinant technology. Authors show that this system offers significant advantages compared to conventional biopanning methods.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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