BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1616

Peptides:

Sequences

Affinity Values

RGDKSRPPVWYVEGE(7)
VQRGAWRWPVVTNE(4)
PAGRQARRPATVPEG(1)
PEWWLEEPPFISSGK(1)

[1.085 ± 0.011, 37.181 ± 5.147, 1.069 ± 0.051, 0.026 ± 0.005]
[0.242 ± 0.017, 10.291 ± 1.133, 1.083 ± 0.031, 0.012]
[0.074, 12.289, 1.013 ± 0.016, 0.025]
[0.199 ± 0.014, 4.433 ± 0.346, 1.083 ± 0.041, 0.005 ± 0.002]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20708963

Target Name:

Anti-CPS monoclonal antibody 13D9

Template Name:

Capsular polysaccharide, CPS

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 PC89 phage display library

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The affinities of selected phages to anti-CPS monoclonal antibody 13D9 were measured by phage ELISA, phage UltramicroELISA (UMELISA), phage sandwich ELISA and inhibition ELISA. In phage ELISA, the absorbance at 492 nm was determined. The absorbance of the control phage PC89 was 0.086 ± 0.011. In UMELISA, fluorescence units (FU) were read in a photometer-fluorimeter plate reader PR-521. The fluorescence units of the control phage PC89 were 2.436. In sandwich ELISA, the absorbance at 492 nm was determined. The absorbances of the phage 2L-18, behaving as the control phage PC89, and PC89 were 0.087 ± 0.020 and 0.077 ± 0.020, respectively. In inhibition ELISA, the absorbance at 492 was determined. The absorbances of the phage 2L-18, behaving as the control phage PC89, and PC89 were 0.119 ± 0.008 and 0.188 ± 0.006, respectively. All data were expressed as means ± SD and were reproduced from the graph.

Brief Description:

BiopanningDataSet Comments:

Immunization of mice with the phage-displayed peptides elicited anti-peptide IgG antibodies.

Curator:

Curation Date:

0000-00-00

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