BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1610

Peptides:

Sequences
GAGNHPDAVSYPDILVKPRL(2) TAYTTGIRWSSQLQRLQHAS(1) MPHLDRVLWKPSGIRDLVSE(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 14500347

Target Name:

CD19+ cells

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

ON543 phage display library (X20)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Cells from different patients were used at the indicated rounds of selection (e.g., R1 = round 1). Selection target referring to the patient: R1, patient 25; R2, patient 16; R3, patient 18; R4, patient 43. Clearing with autologous EBV-immortalized cell line was applied in rounds 2-4. The cell were incubated for 1 h with the phage at 37°C. Cell surface phage were recovered by two acid elutions. The acid fraction was neutralized.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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