BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1568

Peptides:

Sequences
GSMSPYVRWYTP(9) KDASSFQMRPLS(2) SVSVGMKPSPRP(2) KAQSPWSNVDAW(2) HSLKHTQMSYSS(2)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11923297

Target Name:

Anti-LOS monoclonal antibody 9-2-L379

Template Name:

L3,7,9 lipooligosaccharide, LOS

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The antigenicity of the enriched phage clones against mAb 9-2-L379 was tested by ELISA. Absorbance was measured at 450 nm (data not shown). Phage clones expressing the identified linear 12-mer consensus peptides showed no reactivity with mAb 9-2-L379.

Brief Description:

The fourth round of panning was performed at 50 μg/ml of the target antibody 9-2-L379 coating the microtiter plate wells.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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