BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1535

Peptides:

Sequences
GRQYYEGRKPDYRAA(19)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21573118

Target Name:

Anti-HMW-MAA monoclonal antibody VT80.12

Template Name:

High molecular weight melanoma-mssociated antigen, HMW-MAA

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 PC89 phage diaplay library

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

In ELISA inhibition experiments, the peptide GRQYYEGRKPDYRAAC inhibited the binding of the mAb VT80.12 to the HMW-MAA up to 93%.

Brief Description:

BiopanningDataSet Comments:

Twenty individual phage clones were tested for their ligand specificity in phage ELISA. Among these, nineteen phage clones specifically bound to the mAb VT80.12. DNA sequencing of these 19 phage clones yielded one peptide sequence GRQYYEGRKPDYRAA. Peptide inhibited the binding of mAb VT80.12 to the HMW-MAA.

Curator:

Curation Date:

0000-00-00

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