BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1510

Peptides:

Sequences	Affinity Values
FSLSKPP(21) STQAMFQ(9) HSMQLST(4) HAIYPRH(3)	[0.468 ± 0.011] [0.523 ± 0.047] [0.436 ± 0.047] [0.782 ± 0.022]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12951773

Target Name:

A549 bronchial epithelial cell line

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

In phage ELISA, absorbance at 420 nm was measured. Data shown were reproduced from the bar graph and expressed as means ± standard deviations. The absorbance of the negative control phage was 0.379 ± 0.024. Results demonstrated the isolated phage displaying HAIYPRH, STQAMFQ, and FSLSKPP show significantly increased levels of binding to A549 cells compared with a control wild-type phage displaying no peptide on its surface coat.

Brief Description:

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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