BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1507

Peptides:

Sequences
REDLFDRYAHLRTPV QTNVKYDRFDWLLRQ KYDRFVIYKDGSVQT ELREEYKQLGYTGPH

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 8956081

Target Name:

Anti-sperm whale myoglobin polyclonal antibody

Template Name:

Myoglobin

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Phages displaying a ligand for the biotinylated polyclonal antibodies are captured on the dish by binding of the biotin moiety to immobilized streptavidin.

BiopanningDataSet Comments:

Twenty-six samples were sequenced after two rounds of screening. It was found that except for 19% (5/26) of the positive clones containing the insert sequence of D-R-F, there were a few dominant sequence motifs. However, with the help of the Blitz electronic mail server, four sequences were found to show homologies to the myoglobin sequence. Three of the insert peptide sequences (clones REDLFDRYAHLRTPV, QTNVKYDRFDWLLRQ and KYDRFVIYKDGSVQT) showed similarities to amino acid residues 42-50 of the myoglobin sequence, On the other hand, the peptide insert sequence of clone ELREEYKQLGYTGPH was similar to amino acid residues 141-153 of the myoglobin sequence (which was located in the C-terminus of the protein).

Curator:

Curation Date:

0000-00-00

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