BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1505

Peptides:

Sequences
RFDRLK(8) LELFRR(4) RFDWLK(1) GFDRLK(1) LELFRG(1) LGLFRR(1) RCDRLM(1) RVDRLM(1) LEVCSE(1) GCDLYI(1)

Sequences Features:

Motif:

RFDRLK, LELFRR, DRLM

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 8956081

Target Name:

Anti-sperm whale myoglobin polyclonal antibody

Template Name:

Myoglobin

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f3-6mer phage display library (X6)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Phages displaying a ligand for the biotinylated polyclonal antibodies are captured on the dish by binding of the biotin moiety to immobilized streptavidin.

BiopanningDataSet Comments:

Two motifs show homology to the sperm-whale myoglobin sequence: RFDRLK, which matches amino acid residues 45-50 (RFKHLK), and LELFRR, which matches amino acid residues 135-140 (LELFRK).

Curator:

Curation Date:

0000-00-00

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