BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1487

Peptides:

Sequences
THRPPMWSPVWP(8)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11277922

Target Name:

Transferrin receptor protein 1

Template Name:

Iron-loaded human transferrin

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In fact, the target is CEF+hTfR cells expressing the human transferrin receptor. The original phage library was applied to CEF cells. Unbound phage were transferred to another well of CEF cells, before transferring the unbound phage to a well of CEF+hTfR cells.

BiopanningDataSet Comments:

In the final round of biopanning selection, the THRPPMWSPVWP phage represented 89% (8/9) of the phages isolated and sequenced. Phage containing the sequences THRPPMWSPVWP were inhibited from binding the hTfR in a dose-dependent fashion when peptides of the same sequence were present in a competition assay. Interestingly, transferrin did not compete with either of these sequences for receptor binding, suggesting that these peptides bind a site on the hTfR distinct from the transferrin binding site.

Curator:

Curation Date:

0000-00-00

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