BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1477

Peptides:

Sequences

Affinity Values

QLVTDEL(3)
EHQLNRA(2)
YHQTTIP(2)
SLTTDEL(1)
GHQVSRL(1)
HGLHLPV(1)
VLPDSVW(1)
SLPTLTL(1)
KIPIALS(1)
HAIYRPH(1)
FHQMPDA(1)
ADCTTPT(1)
FGGDADR(1)
SGIAVNP(1)
HSYTLMF(1)
GHWTRFA(1)

[0.18]
[NT]
[NT]
[0.20]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 9880034

Target Name:

Anti-CMV CP polyclonal antibody

Template Name:

Capsid protein, CP

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding of the positive clones to Fny-CMV polyclonal antibodies was confirmed by ELISA. Absorbance at 405 nm was determined and average ELISA value was shown. The average ELISA value of the positive clones was at least three times as high as that of a negative control clone (with average A405 of 0.06). These results suggest that the sequence LXTDEL, corresponding to amino acid residues 194-199, is a dominant epitope in the Fny-CMV CP. NT denotes not tested.

Brief Description:

BiopanningDataSet Comments:

Nine of 19 clones (47%) from the third round of biopanning carried insert sequences resembling a sequence of the CMV CP.

Curator:

Curation Date:

0000-00-00

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