BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1466

Peptides:

Sequences
NSSPYLNTK(20) ASSLLASSP(16) PQSPGSSFP(10) PHNRQESPA(7) IPFPTLFAP(2) PHESDATVR(2) LVGTPNKTK(2) PSPSLSHPL(1) PPLKPVIDE(1)

Sequences Features:

Motif:

S(2)-[PLF]

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 7994916

Target Name:

Anti-TNF-α polyclonal antibody

Template Name:

Tumor necrosis factor

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

pVIII-9aa phage display library (X9)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Biotinylated anti-human immunoglobulin was added to the reacted library for 3 h at room temperature and the immune complexes were immobilized in streptavidin-coated polystyrene tubes.

BiopanningDataSet Comments:

Authors demonstrated that three synthetic peptides (ASSLLASSP, NSSPYLNTK and PPLKPVIDE) displayed by the selected phages reduced the binding of the autoantibodies to TNF-α protein by 50%. Interestingly, the sera of mice (BALB/c) immunized with phages displaying ASSLLASSP and NSSPYLNTK peptide showed an anti-TNF-α response as detected by ELISA. This response was not found in mice immunized with the wild type phage. Thus, the recombinant phages selected from the phage libraries could be used as carrier for immunization, and therefore as a tool for vaccine development.

Curator:

Curation Date:

0000-00-00

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