BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1458

Peptides:

Sequences	Affinity Values
SQSYPTRNS DQSYPADAN GGSQSYPDL EDGGAQSYP WRTAPESYP VQATLQSYP IHQSYPDRG REGAIQSYP RLTPESDDR QARYAKEPD	[1.006 ± 0.021] [NT] [NT] [NT] [NT] [NT] [NT] [NT] [0.662 ± 0.025] [0.595 ± 0.042]

Sequences Features:

Motif:

QSYP, R-x(2)-P-E-P-D

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12545550

Target Name:

Anti-gp120 monoclonal antibody F105

Template Name:

Surface protein gp120, SU

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

J404 phage display library (X9)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Plates were coated with MAb F105 and negative control MAb F91 at 0.1 μg/well. Phage clones were added at 1.0e9 pfu/well. Parent phage M13KBst was a negative control. Phage binding was detected by rabbit anti-M13 antiserum, followed by AP-conjugated goat anti-rabbit secondary Ab. Absorbance values at 405 nm are reproduced from the bar graph and shown as the mean of duplicate samples ± standard deviations. The absorbance for the phage M13KBst was 0.053 ± 0.007. NT denotes not tested.

Brief Description:

Bound phages were eluted by the addition of rgp120.

BiopanningDataSet Comments:

Phage display selection against MAb F105 resulted in a number of phage clones that matched the QSYP consensus sequence. Many phage clones selected by MAb F105 also matched the consensus RXXPEPD.

Curator:

Curation Date:

0000-00-00

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