BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1429

Peptides:

Sequences
LEKGTPW AAKGTPW EPDKMTT EPDKSII QKTAASY GKQPASD AVTFSKG

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16199263

Target Name:

Polyclonal antibody IgE from birch pollen-allergic patient #1

Template Name:

Major pollen allergen Bet v 1-A

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Two millilitre aliquot of a tosyl-activated M280 Dynabeads suspension were loaded with rabbit-anti-human IgE , and blocked with skim milk in PBS. Patient serum (600 μl) was diluted in 9.4 ml PBS with 0.05% Tween 20 and 0.1% skim milk, and incubated overnight at 4 ℃ with 200 μl coated beads suspension. In the second round, phages bound to the beads were eluted with Bet v 1.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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