BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1420

Peptides:

Sequences

CDRRACRVAFSSRTG
LVFSLFVILLLVLGV
FGFFSSNPLVHVHWD
FIPAYCCVVCAYLSV
GYFNCLVVHRHALRC
LSSLCGLPFGRPQCV
SFARLLFRLCLTTTP
FFFGGLRLLVLLRES
FFYRLVFCHYPRLVF
DCSRFSWLSSVFAVA
HLYGFSLIMFCMVTI
SLVLFTSNVYPMFVF
WDSSCFVPFLCSGSS
CSDIVVASPSPFPTC
CVLSLCLFMVLFFSV
FFLPVGFRAFLLGRR
FVDSCLLCTFTTLRT
LFVLSSSDAHECSAY
RCVLSYGPNVFQPVD
SFCISCLCVCGGDGR
SFDAALCLVSSLFGA
TGSHCGVYKVGLGVV
VTYFTWSFDPRGCCW
VVVIVFLCLVWLVGG
YVLWWRLLFFILIIS
YYAIFFHRVLRMYSV
SFDFGSFDFVSPLAP
CIDVNCFVWTFGMPF
FGYELSFLSSWSVFG

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18930404

Target Name:

Irinotecan, Iri

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 T7 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The screening procedure involved a cuvette type 27-MHz quartz-crystal microbalance (QCM) apparatus with introduction of self-assembled monolayer (SAM) for a specific small-molecule immobilization on the gold electrode surface of a sensor chip. For Iri-bio immobilization, the gold sensor surface was initially coated by treatment with 100μl of neutravidin solution (100lg/mlaq) for 30 min at room temperature. After washing the gold electrode surface, Iri-bio was applied and left for 16 h under a humid and shaded atmosphere at room temperature. The gold surface was thoroughly washed prior to conducting the screening experiments.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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