BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1412

Peptides:

Sequences

NTAHSDMHSP(2)
KAEERFHEVR(1)
WNIFQSLSSP(1)
RVAATAAREE(1)
TPEPLEEAAS(1)
DHVFKRPQPS(1)
ETCVEKNEAD(1)
WRRDGC(1)
YMDPHTQREA(1)
EPVRDNCAPS(1)
VERDIATRPW(1)
ERPEIEDVCQ(1)
QKTLNTSNAN(1)
VKTLNTSNAN(1)
RDTTMWEVNA(1)
WGHCSQGMIE(1)
PPFDVFHNPM(1)
DTHAGMHSPT(1)
RMPHHDPQLM(1)
TQNLMQMQHA(1)
SPFMLMHGEH(1)
TCQAGRESMHNP(1)
WETMHNPGTP(1)
DALNMHEGRP(1)
SKLETTMHSP(1)
LGGMDSMHSP(1)
MAQMHEPVRS(1)
LPQHNMMHDP(1)
PSNYSAMHAP(1)
RCETHFNMHEPY(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 19390580

Target Name:

Anti-HER-2 extracellular domain polyclonal antibody

Template Name:

Extracellular domain of HER-2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

XC(X)10CX phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The library was initially depleted of phages recognized by naive mouse serum by 3 sequential pannings of the library with immobilized serum of non-immunized mice. The resultant \"depleted\" library was used for mimotope identification. Similarly, the sera obtained from immunized mice were initially depleted of anti-phage activity by overnight incubation at 4 ℃ with wild type phage immobilized on plastic plates. The sera were also depleted of IgM by similar incubation with immobilized anti-IgM antibody.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

First |

Prev |

1412

| Next

| Last