BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1410

Peptides:

Sequences

Affinity Values

CKSSGKLISLC
CGTKLVCFAAC
CCNGRLYCGPC
CCAGGLTCSVC
CSGRLYCHESWC

[14.98 ± 2.51, 11.73 ± 1.78]
[12.72 ± 2.11, 14.09 ± 2.18]
[13.87 ± 2.07, 1.02 ± 0.00]
[13.73 ± 2.64, 12.50 ± 1.30]
[18.74 ± 1.30, 10.23 ± 1.30]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10229859

Target Name:

Anti-HIV-1 polyclonal antibody

Template Name:

Human immunodeficiency virus type 1, HIV-1

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

pVIII-9aa.Cys phage display library (CX9C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Comparison of ELISA reactivities of phagotopes with sera of LTNP vs AIDS subjects was performed by ELISA. The difference between OD405 nm and OD620 nm was determined by an ELISA reader. Data are expressed as fold increase of the average values of the phagotopes over values of the wild-type phages and as mean ± SEM of fold increase for each phagotope. Data shown are reproduced from Fig. 1B in the reference. Data in the first column are values of phagotopes with sera of LTNP. Data in the second column are values of phagotopes with sera of LTNP. Values were considered positive when at least 4-fold higher than the background signal of wild-type phage.

Brief Description:

In the immunoaffinity selection, serum IgG was linked to magnetic microbeads previously coated with an anti-human (Fc-specific) polyclonal Ab of beads suspension. Given the fact that sera from long-term nonprogressor (LTNP) subjects show higher titers of neutralizing Abs than sera from AIDS patients, initial screening was performed with LTNP sera.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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