BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1345

Peptides:

Sequences
TYTDWLNFWAWP(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21297616

Target Name:

Myelin basic protein, MBP

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In the selection against MBP, phage expressing a library of peptides were selected through multiple cycles for binding to biotinylated MBP, using avidin agarose to isolate selected phage. Specifically, the phage library was mixed with biotinylated MBP and allowed to bind for 1 h. Avidin agarose was added and incubated for an additional hour. Nonbinding phage were removed by washing the agarose three times with PBS solution and the supernatant was plated for titer and amplification for subsequent cycles.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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