BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1341

Peptides:

Sequences
WHWRNPDFWYLK(0.225) WHWTWLSEYPPP(0.215) LETSKLPPPAFL(0.125) WHWSQWLSGSPP(0.085) WHRTPQFWAFPW(0.07) SVSVGMKPSPRP(0.05) WHKTPWFWPTNL(0.05) WHWSWQPQRHSP(0.04) WHWQYTPWWRGS(0.03)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21329494

Target Name:

Gliadin

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Panning was done both with gliadin dissolved in urea and in NaHCO 3. Since electrostatic binding of the phage to the target protein weakens with increasing ionic strength, which in turn influences the specificity of the interaction, different buffers with different ionic strengths were tested prior the actual panning experiments.

BiopanningDataSet Comments:

Although identical sequences were frequently picked up in independent experiments, altogether more than 160 unique sequences encoding peptides with potential gliadin binding activities were identified. In order to confirm that the selected phage clones interacted with gliadin proteins, nine different phage populations that had repeatedly been picked up in different panning experiments were chosen. Together, these nine sequences represent 89% of all identified gliadin-binding sequences.

Curator:

Curation Date:

0000-00-00

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