BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1338

Peptides:

Sequences
CVRPFAL(5) CVRAPTL(2) TVRPFTL(2) TLWDLIP(2) TLWPLSP(2)

Sequences Features:

Motif:

TLW, VRP

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21278676

Target Name:

Modified helix 31 of bacterial 16S ribosomal RNA

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

A separate counter selection was also carried out in the third round of selection against wild-type h31 in which free unmodified h31 was added to the phage and modified h31-streptavidin mixture.

BiopanningDataSet Comments:

One hundred phage isolates were sequenced after the third round of selection. Only peptide sequences with repeated sequences or common motifs are shown. Several of the peptides exhibited moderate affinity (in the high nM to low μM range) to modified helix 31 in biophysical assays, including surface plasmon resonance (SPR), and were also shown to bind 30S ribosomal subunits. These peptides also inhibited protein synthesis in cell-free translation assays.

Curator:

Curation Date:

0000-00-00

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