BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1242

Peptides:

Sequences
GRFLIRVTSSPLGPD(1) TGSGLYLHQMVYLYQ(1) FLIDSPLASIGPTSM(12) FMIDSPLASIGPTSM(2) VIDLSGTRKSSSGTM(1) VRKTTSHPPSYALLH(1) TPPYRAALATPVLLL(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20682764

Target Name:

Campylobacter jejuni

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f88-15mer phage display library (X15)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The phage-display library was ubtracted twice against C.jejuni strain ACM 3393 in 3 ml PBSg in a Petri dish, prior to being added to C.jejuni strain C338. Non-binding phages were washed away using PBSg as the wash buffer. Cell surface bound phages were recovered with a low pH elution buffer and amplified by infection in E.coli K91BlueKan cells and used in subsequent rounds of affinity selection. In the second and third rounds of affinity selection, non-binding phage were washed away with PBSg.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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