BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1212

Peptides:

Sequences	Affinity Values
SYSWMYE(1400) YWDGQW(22) WWDGQF(13) YDWLYE(6) DYAWMY(2)	[-, 0.69] [-, 0.20] [-, 0.17] [-, 0.42] [-, 0.67]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 8760499

Target Name:

Anti-GXM monoclonal antibody 2H1

Template Name:

Glucuronoxylomannan, GXM

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f3-6mer phage display library (X6)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Large-scale phage preparations were made using PEG precipitation. The direct ELISA was done by absorbing anti-phage antibody to the ELISA plate and then adding 2e10 phage. 2H1 (2 μg/ml) was then added and the ELISA was developed with an alkaline phosphatase-conjugated anti-γ1 antibody. The enhanced direct ELISA was carried out the same way with a threefold excess of anti-γ1 antibody (6 μg/ml) added together with 2H1.

Brief Description:

For the hexapeptide library, complexes between 2H1 and phage were captured with streptavidin-coated magnetic beads (Advanced Magnetics, Inc., MA).

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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