BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1199

Peptides:

Sequences
ILPEVVS(1) TLPEVLS(1) QLPEVSS(1) ALPEVLS(1) MLPEVLP(1) LELPEVM(1) VLQEVSS(1) AFPSPTL(1) AYTSAHK(1)

Sequences Features:

Motif:

LPEV

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 9598980

Target Name:

Anti-syndecan-1 monoclonal antibody B-B4

Template Name:

Syndecan-1, SYND1

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

B-B4 was produced by ascitic fluid and purified on a protein A column. Coated mAb was used to select the phages which were then eluted with an acidic treatment, and amplified in bacteria for the next round of selection. After the third round of selection bound phages were eluted by adding the selecting mAb as competitor and then acidic conditions were applied to recover the remaining phages.

BiopanningDataSet Comments:

Independently of the elution procedure used, the number of recovered phages was at least 30 times higher from the well coated with the selecting mAb than with an irrelevant control mAb, suggestting that the selected phages were mAb specific.

Curator:

Curation Date:

0000-00-00

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