BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1185

Peptides:

Sequences
CNVSDKSC(13) CNKTDKGC(2) CNWTDKTC(1)

Sequences Features:

Motif:

N-x-[ST]-D-K-[ST]

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12496764

Target Name:

Purified IgGs from serum of prostate cancer patient 3

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX6C phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

First, a pre-clearing step was used to remove nonspecific clones by pre-absorbing the phage peptide library onto purified IgGs from normal serum pooled from volunteer agematched blood-donor control men. Next, the pre-cleared phage library was selected onto the pool of IgGs purified from the serum of prostate cancer patients.

BiopanningDataSet Comments:

Authors identified a consensus motif, NX[ST]DK[ST], that bound selectively to circulating antibodies from cancer patients over control antibodies from blood donors. Authors validated this motif by showing that positive serum reactivity to the peptide was specifically linked to disease progression and to shorter survival in a large patient population.

Curator:

Curation Date:

0000-00-00

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