BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1179

Peptides:

Sequences	Affinity Values
LPPSIPFGLTVG(16) LLPSIPNQAYLG(14)	[5.63] [6.50]

Sequences Features:

Motif:

L-x-P-S-I-P

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12474050

Target Name:

Anti-JL1 leukemia-specific monoclonal antibody

Template Name:

JL1 antigen

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Surface plasmon resonance (SPR)

Affinity Measurement Description:

The interaction between anti-JL1 mAb and the mimetic peptides was tested using a surface plasmon resonance biosensor. The Kd (μM) value was measured and shown.

Brief Description:

The phage library was pre-incubated with an irrelevant mAb (IgG1, kappa) for 30 min and then added into the well coated with anti-JL1 mAb.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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