BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1154

Peptides:

Sequences
QHFNNSVNLGFT(20) SPERHLHDLRPY(3) AILIISLMLGFT(1) APTVKNCPSPCP(1) YTTTWRPPYSHN(1) YWWIIIRQYTTP(1) TLATVPSSSLFV(1) LVDHHQTYTTPP(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11121403

Target Name:

BAG family molecular chaperone regulator 1

Template Name:

Heat shock cognate 71 kDa protein

Structure of Target-Template Complex:

1HX1

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The recombinant Hap46 was immobilized on Petri dishes, blocked, and incubated with phage library following the manufacturer’s protocol. To eliminate enrichment of phage clones interacting with the plastic surface or the blocking agent, the following two strategies were employed: (i) eluted and amplified material from the first panning was either subjected to a preadsorption step on bovine serum albumin coated Petri dishes prior to subsequent pannings, or (ii) bovine serum albumin as blocking agent was alternated with casein in the form of dried milk powder.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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