BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1131

Peptides:

Sequences
ELRGDSLP(10) EVRGDSLP(2) VDLPSHPE(1) VNLPEHPE(2) VDLPRSDT(1) HTTKEQMA(1)

Sequences Features:

Motif:

V-[DG]-L-P-[ET]-H

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 14617786

Target Name:

RG2 rat glioma cells

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f8-8mer phage display library (X8)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

RG2 cells were grown to a sub-confluent monolayer. First, an aliquot of the primary library in washing/blocking buffer was added to an empty flask (depletion flask) to remove plastic-binding phage clones. Buffer containing phage that did not bind to plastic was transferred to the flask with RG2 glioma cells for incubation. After that, phage not associated with tumor cells were washed away. Cell-surface bound phage were recovered with lysis buffer.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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