BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1118

Peptides:

Sequences	Affinity Values
SWTPDKWLRPSG(2) NIAHPDRWDRLR(1) ADNPIKWDRART(1) LSNTANPWSWDR(1) ASNWTDFRVTDR(1) GMLPPQLFDRHH(1) AEPFYSWDRASR(1)	[3.14/3.03] [3.14] [3.26] [3.02] [2.97] [2.91] [3.07]

Sequences Features:

Motif:

P-x(2)-W-D-R

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12902494

Target Name:

Monoclonal antibody HC-10

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Reactivity of phage particles with mAb was measured by ELISA. Absorbance was read at 492 nm with a Multiscan microplate reader (Benchmark; Bio-Rad).

Brief Description:

At each round, phage particles binding to isotypic and allotypic determinants of mAb HC-10 were removed by a preadsorption step on mAb HC-10 isotype-matched mAb W6/32.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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