BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 111

Peptides:

Sequences

Affinity Values

KGSHEALHRHIR
KPHSTHLPAHQP
HTIHSKQSHKNS
HSKLNNRHHALL
TPHSKPHFHKLP
HSRHNHYLHPWA
HSKHTNHPPVGS
MPKHIHRPHNIN
WTVLPHEGGLSS
LPSRHAAHPHVR
KSLSRHDHIHHH

[0.556, 0.953]
[0.255, 0.795]
[1.920, 1.206]
[0.803, 1.112]
[0.193, 0.876]
[0.477, 0.974]
[0.260, 0.849]
[1.184, 1.007]
[0.473, 1.381]
[0.351, 0.906]
[0.577, 0.775]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 15041534

Target Name:

Anti-hTERT polyclonal antibody

Template Name:

Telomerase reverse transcriptase

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding of anti-hTERT polyclonal antibody with selected phages was quantified by both direct ELISA and sandwich ELISA methods. The absorbance was measured at 490 nm. Data shown were reproduced from the graph.

Brief Description:

In each round, a subtractive panning was performed on normal mouse IgG.

BiopanningDataSet Comments:

After 3 rounds of screening, 13 of 24 randomly selected phage clones were identified as positive clones that could specifically bind to anti-hTERT anibody but not to normal mouse IgG.

Curator:

Curation Date:

0000-00-00

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