BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1103

Peptides:

Sequences
MANPGRA MQPSLVH MTTYPLS MTTTMQL MAVDPLR

Sequences Features:

Motif:

M-x(2)-P

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12032665

Target Name:

Fibroblast growth factor receptor 2, FGFR-2

Template Name:

Heparin-binding growth factor 2 (HBGF-2)

Structure of Target-Template Complex:

1EV2

1II4

1IIL

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The phage library was incubated with 911 human embryonic retinoblast cells for 1 hour at 4℃. Then medium containing unbound phage was removed and discarded. Finally, phage bound to cells were specifically eluted by the addition of FGF2 (in PBS) to each well with gentle agitation.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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