BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1066

Peptides:

Sequences	Affinity Values
WTYIPPV WEYIPNV WSYIPVV FNYVPPT FTYIPNS FTYIPHT FDFNRRI TAPGVST	[High] [Very high] [High] [High] [High] [ND] [High] [Low]

Sequences Features:

Motif:

[FYW]-[RNDCEQHKSTY]-[FYW]-[AGILMFPWV]-P

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 14506253

Target Name:

SurA (delta-P2)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Contents in the square bracket were based on results of ELISA affinity evaluations. ND represented not determined.

Brief Description:

Mature E. coli SurA protein was expressed as a fusion with a self-cleaving intein.

BiopanningDataSet Comments:

Authors find that both full-length SurA and its core module have similar peptide bind-ing specificities and affinities for peptides with a consensus sequence of the form aromatic-polar-aromatic-nonpolar-proline.

Curator:

Curation Date:

0000-00-00

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