BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1063

Peptides:

Sequences
FEGFSFLAFEDFVSSI(10)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12963036

Target Name:

Intercellular adhesion molecule 1, ICAM-1

Template Name:

Integrin alpha-L beta-2

Structure of Target-Template Complex:

1MQ8

3TCX

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X16 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

To assure a native presentation of the extracellular domains of ICAM-1 we have transfected COS-7 cells with plasmid inserted with cDNA encoding the common form of ICAM-1. The obtained ICAM-1 positive cell subline was used for the screening of the phage library. During which the library was first subtracted on the nontransfected COS-7 cells in each of the four rounds of panning prior to exposing the library to ICAM-1-expressing cells.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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