BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 105

Peptides:

Sequences	Affinity Values
MHRHAHPMYQPP(3) MHRHYHPWLTPS(2) FHRTPWLQFSLP(2) WPKHFHIPQWWT(1)	[0.814] [NT] [0.887] [1.134]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3/4

Reference:

PMID: 15207087

Target Name:

Anti-hantaan virus monoclonal antibody B11

Template Name:

Hantaan virus

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Selected phage clones were tested by ELISA for their binding specificity to anti-hantaan virus monoclonal antibody B11. The absorbance was determined as A450/A630 nm. Firstly the mAb B11 (100 mg/L) was incubated with phages. When the titer of mAb diluted 400 times, the absorbances were measured and shown. NT denotes not tested.

Brief Description:

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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