BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1007

Peptides:

Sequences
YMRYYESSLKSYPDW(16) TMTFPENYYSERPYH(2) PPPIFRYYEYWPTSY(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 8954559

Target Name:

Alpha-bungarotoxin

Template Name:

Acetylcholine receptor subunit alpha

Structure of Target-Template Complex:

1IDG

1IDH

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 M13 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

HPLC-purified alpha-bungarotoxin from the snake venom of Bungarus multicinctus was biotinylated and attached to an immobilized nitrostreptavidin matrix. Nitrostreptavidin reversible biotin-binding properties was prepared by chemical modification of the protein using tetranitromethane. Bound phage were eluted with 0.1M HCl titrated to pH 2.2 with glycine.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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