General information | |
ACovPid: | ACoVP100181 |
Trivial Name: | HKU4-HR2P1 |
Amino Acids Sequence: | GPNFAEISKINTTLLDLSDEMAMLQEVVKQLNDSYI |
Length: | 36 |
C-Terminal Modification: | None |
N-Terminal Modification: | None |
Chemical Modification: | None |
Peptide Source: | Tylonycteris sp. Bat coronavirus HKU4 : 694007 |
Source Description: | This article use the piptide from Tylonycteris sp. Bat coronavirus HKU4, but the source article of Bat coronavirus HKU4 is 《SARS-like virus in the Middle East: A truly bat-related coronavirus causing human diseases》(PMID:23143870) |
Against Virus: | |
Inhibition Value Type: | IC50 |
Inhibitory Effect: | 2.72 |
Inhibitory Unit: | µM |
Target Domain Name: | |
Assay: | Pseudotyped virus infection inhibition assay |
Assay Description: | The package of MERS-CoV pseudovirus was performed described in our previous studies (PMID: 24473083, 26164863). Briefly,we cotransfected 293T cells with plasmid pcDNA3.1-MERS-S with or without Q1020H/R mutationand plasmid pNL4-3.luc.R-E encoding Env-defective, luciferase-expressing HIV-1.Then,the pseudoviruses in the supernatant were collected 48–72 h post-transfection and quantified bythe level of lentivirus p24. Target cells, Huh-7, were preplated in 96-well plates (1×10^4cells per well).The MERS-CoV pseudovirus was premixed with peptide at indicated concentration and incubated for30 min at 37◦C. PBS was used as no inhibition control. Then, the mixture was added to the Huh-7 cells,replaced by fresh medium 12 h post-infection, and then incubated for an additional 48 h. TransducedHuh-7 cells were lysed for detection of relative light units (RLU) according to the luciferase assaysystem manual (Promega, Madison, WI, USA) |
Anti-CoV activity in vivo: | |
Reference: | 30646495 |
Comment: | The source of Q1020/Q1020H/Q1020R : Huh-7 and 293T cells were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai,China). All cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10%fetal bovine serum (FBS). Plasmids, including pcDNA3.1-MERS-S, pcDNA3.1-MERS-S with Q1020Hor Q1020R mutation, pAAV-IRES-S-GFP and pNL4-3.Luc.R-E, were constructed in our laboratory.All peptides were synthesized by KareBay Biochem (Monmouth Junction, NJ, USA) with HPLCpurity >90% [21,22]. The average hydrophilicity of peptides was predicted by using an onlinepeptide-calculator program (available online: http://www.bachem.com/service-support/peptide-calculator/). |
3D structure: | |
Structure Experiment Verified: | NO |
Similar Peptides: | ACoVP100183   ACoVP100141   ACoVP100187   ACoVP100116   ACoVP100070 |