Screening the random peptide libraries constructed with phage display provides an efficient and convenient approach to acquire mimotopes. However, target-unrelated peptides creep into mimotopes from time to time because corresponding phage clones may grow faster or bind to contaminants or other components of the screening system. SAROTUP is a suite of tools that can scan, report and exclude possible target-unrelated peptides from the noisy experiment results of phage display. As freely accessible service for the data preprocessing and cleaning of phage display, you are welcome to use these tools in your work.
If you use tools in the SAROTUP suite, please cite the following references:
1. Huang J, Ru B, Li S, Lin H, Guo FB. SAROTUP: scanner and reporter of target-unrelated peptides. Journal of Biomedicine and Biotechnology 2010; 2010: 101932. [PMID: 20339521; Download: fulltext pdf ]
2. He B, Chai G, DuanY, YanZ, Qiu L, Zhang H, Liu Z, He Q, Han Ke, Ru B, Guo FB, Ding H, Lin H, Wang X, Rao N, Zhou P, Huang J. BDB: Biopanning Data Bank. Nucleic Acids Research 2016; 44(D1): D1127-D1132. [PMID: 26503249; Download: fulltext pdf]
3. Huang J, Ru B, Zhu P, Nie F, Yang J, Wang X, Dai P, Lin H, Guo FB, Rao N. MimoDB 2.0: a mimotope database and beyond. Nucleic Acids Research 2012; 40(Database issue): D271-D277. [PMID: 22053087; Download: fulltext pdf ]