To predict the protein interaction site based on mimotopes is a fascinating work for computational biologists. Quite a few methods and tools such as SiteLight, 3DEX, MIMOP, MIMOX, Mapitope, Pepsurf, Pepitope, Pep-3D-Search, and Episearch etc. have been developed in recent years. Benchmark datasets are desperately needed for tool evaluation and new algorithm development. MimoBench is a benchmark dataset derived from the BDB database. There are 24 sets of biopanning data for antibody-antigen interactions with complex structure solved, 24 sets of biopanning data with solved structures of corresponding receptor-ligand complex and 31 sets of biopanning data for other protein-protein interactions with complex structure solved. In total, it makes a benchmark with 79 sets of data for mimotope-based protein-protein interaction study. This data set will be not only useful for benchmarking the existing tools, but also helpful for developing new and better algorithm to predict protein-protein interaction sites based on mimotopes. (Click here to download the benchmark data set)