BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1061

Peptides:

Sequences
CLLRMSIC(10) CRLKSRRNC(3) CLPPPNRRC(2) CHTLLKSQC(1) CRRIKPRIC(1) CTMLNRNMC(1) CRLIHLMLC(1) CIRLRXLRC(1) CPISNRLIC(1) CPLMRRILC(1) CRRNRSRKC(1) CRTRPKRRC(1) CKPLMSIRC(1) CRRLRLHRC(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11532073

Target Name:

Intercellular adhesion molecule 1, ICAM-1

Template Name:

Integrin alpha-L beta-2

Structure of Target-Template Complex:

1MQ8

3TCX

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Authors used the mAb 84H10 to elute bound phage, because this mAb has previously been shown to bind ICAM-1 and prevent LFA-1 interaction, implying that LFA-1 and 84H10 bind at similar locations on ICAM-1.

BiopanningDataSet Comments:

Fourteen different peptide sequences were identified and analysis of these sequences did not show a consensus sequence. One peptide sequence (*CLLRMRSIC*) was present on 10 of the 26 sequenced phage.

Curator:

Curation Date:

0000-00-00

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